Enterococcus faecalis sow feed additive performance and immune Zhengzhou Oukebaike biotech companies to explore the effects on performance of sows Enterococcus faecalis and some immune parameters, select the breed, age, body condition, the average parity pregnancy close Yorkshire sows weighing around 150kg 24, were randomly divided into two treatments with established three replicates of four pigs. Two treatment groups were in the control group, fed commercial feed, the test group for Enterococcus faecalis, Commodity dietary 200g / t faecalis, experimental observation from sows prenatal to postnatal 30d 21d, co-51d. The results show that: After adding faecalis in sow diets, their number born alive, the average birth weight, weaning weight compared with the control group were significantly increased (P <0.05), weaning survival rate test group improved compared to the control group (5.56%). Lactation management directly affects the survival rate of piglets before weaning. How to improve the performance of lactating sows focus has always been of concern. Sow prenatal and postnatal inflammatory antibiotics as long-term use of antibiotics, in bringing significant economic benefits, but also led to food safety, drug resistance and immune suppression and other issues. Therefore, the development and promotion of an effective alternative to both antibiotics, hormones and can effectively care, disease prevention, new green growth-promoting feed additives has become a top priority, and that is one of the hotspots of which probiotic research. It is able to secrete organic acids, bacteria, vitamins, digestive enzymes and metabolites, to improve animal intestinal acidic environment, inhibit the growth of harmful bacteria. Optimize the structure of the intestinal flora, stimulate intestinal mucosal immunity. Improve the body's resistance to disease and to improve animal performance purposes. Enterococcus faecalis is the normal intestinal flora of animals facultative anaerobic probiotic lactic acid bacteria, as opposed to strictly anaerobic, cultivation and preservation conditions are harsh Bifidobacterium, Lactobacillus, it is easy to Enterococcus faecalis production and use of preferred species. Many studies show that it has the effect of improving animal performance, improve animal immunity to disease and so on. National Feed Engineering Technology Research Center, nearly one hundred from lactic acid bacteria and resistance test was repeated screening of strong activity in high yield and excellent biological characteristics of Enterococcus faecalis strains, develop livestock applicable Comfort Series . In this experiment, Enterococcus faecalis (trade name: strong Lactasin) for the study, measured by test breeding and production performance indicators, to study its effect on the production performance of sows, as probiotics in livestock industry further provide the basis for the application. MATERIALS AND METHODS Materials Enterococcus faecalis (trade name: strong Lactasin Zhengzhou Oukebaike biotechnology R & D production) by the National Feed Engineering Technology Research Center developed, Zhengzhou Oukebaike Group provides, including Enterococcus faecalis viable count reached 2.0 × 109CFU / g. 1.2 Test animals select species, age, body condition, the average parity, gestational close, weighing about 150kg Large White sows 24. 1.3 Test Design and Management 24 Yorkshire sows were randomly divided into two treatments with established three replicates of four pigs. Two treatment groups were in the control group was fed a commercial feed, the test group for Enterococcus faecalis, Commodity dietary 200g / t faecalis, experimental observation from sows prenatal to postnatal 30d 21d, co-51d. Breeding sow pig bed for the navigation bar, pigs fed ad libitum, automatic drinking water supply, according to the conventional breeding pigs feed grouping rules were feeding. 1.4 Performance indicators measuring the day of farrowing, litter size records, live litter size, litter weight at birth, when the number of records weaned 21d weaning, weaning weight. Use the following formula to calculate the rate Chuhuo earners, average birth weight, weaning weight and average weaning survival. Live Aberdeen rate (%) = number born alive / litter size × 100 = average birth weight of newborn litter weight / average number of pigs per litter weaning weight = weaning weight / litter number of pigs weaned weaning survival rate (% 0,1,3,5 days) = the number of piglets weaned / litter size × 100 1.5 colostrum samples collected after farrowing 3rnl milk samples were collected from different nipple each sow. After sample collection, centrifugal separation of whey and placed at -20 ℃ under test. Whey IgA, IgG and IgM was measured by ELISA kit operating instructions. 1.6 statistical test data "mean ± standard deviation," said the results of the use of Excel and SPSS14.0 statistical software version in the process of One-Way ANOVA analysis of variance, P <0.05 was considered significant. 2 Results 2.1 faecalis impact on sow performance can be seen from Table 1, in this experiment, sow dietary Enterococcus faecalis, which born alive, average birth weight, weaning weight and were significantly increased compared to the control group (P <0.05). At the end of the test sows weaning piglet weaning weight and average weaning weight than the control group increased by 22.70% and 5.30%, weaning survival rate than the control group, the experimental group improved 5.56%. Table 1 in feces enterococci sow performance? Control group test group litter size (head) 10.76 ± 2.98 11.95 ± 2.57 litter size (head) 9.91 ± 2.42a 11.28 ± 2.65b live litter rate (%) 92.10a 94.39b litter weight at birth (kg) 14.80 ± 3.78 15.94 ± 3.25 average birth weight (kg) 1.55 ± 0.23a 1.67 ± 0.24b weaned (head) piglets 9.34 ± 0.62 10.95 ± 0.94 weaning weight (kg) 54.01 ± 13.22a 66.27 ± 9.94b average weaning weight (kg) 5.85 ± 0.51a 6.16 ± 0.67b weaning survival rate (%) 86.80a 91.63b Note: peer data representing the difference between the different letters shoulder note significant (P <0.05), lower table with. 2.2 faecalis impact on sow colostrum immunoglobulin content can be seen from Table 2, when it had just given birth sow colostrum IgG, IgA, and IgM levels are high, one day after a sharp decline in the birth, 5 days after birth was close to the level of ordinary milk. Among them, 0 days test group sow colostrum IgG and IgA levels were compared with the control group improved 14.18% and 35.51% (P <0.05); delivery one day test group sow colostrum IgG and IgA levels were compared with the control group improved 28.76% and 47.84% (P <0.05). ? IgG (mg / ml) IgA (mg / ml) IgM (mg / ml) in the control group test group control group test group test group 0 78.91 ± 8.01a 90.10 ± 15.48b 10.42 ± 1.57a 14.12 ± 2.77b 4.77 ± 1.52 5.04 ± 1.11 1 26.18 ± 6.25a 33.71 ± 12.05b 5.33 ± 1.16a 7.88 ± 1.81b 2.27 ± 0.51 2.04 ± 0.37 3 12.38 ± 2.35a 15.86 ± 6.04b 2.88 ± 0.78a 3.80 ± 1.30b 0.97 ± 0.52 0.87 ± 0.57 5 0.41 ± 0.17 0.52 ± 0.18 0.12 ± 0.05 0.16 ± 0.04 0.02 ± 0.01 0.01 ± 0.01 Table 2 faecalis impact on sow colostrum immunoglobulin content of 3 3.1 faecalis impact on the performance of the sow 20 days ago, the piglet weight gain nutrients needed almost entirely provided by the sow's milk, 21-day-old piglet weaning weight is not only reflected lactation performance of sows and piglets while future growth performance and growth rate are very impact. The results showed that after adding feces enterococci in sow diets, compared with the control group, the experimental group, the average birth weight of piglets, the average weaning weight were significantly increased. This shows that in sow diets adding feces enterococci can promote, directly or indirectly related to hormone secretion and lactation, and enhance the development of the mammary gland secretory activity, significantly improve the performance of lactating sows lactating. This may be due to feces enterococci can reduce to some extent the production of sows stress, enhance immune function and promote uterine recovery, help restore physical sows and gilts intestinal flora balance by adjusting improve feed efficiency, promote the absorption of vitamin B, vitamin A, vitamin E and other synthetic ingredients in favor of milk, thereby increasing the growth performance of piglets. 3.2 faecalis sow colostrum immunoglobulin content of colostrum IgG is the major immunoglobulin content in colostrum is often 50-150 times the milk. IgA secreted into serotypes and IgA, the body of the local mucosal immune respiratory, digestive, etc. has anti-inflammatory effects. Before weaning maternal IgG piglets through colostrum into the blood circulation, provide humoral immunity, and three days after the birth mother's milk in the main immunoglobulin IgA in the intestinal mucosa SIgA adhesion responsible local immunity. The results showed that after adding in sow diets feces enterococci, compared with the control group, the experimental group during childbirth and after childbirth sow colostrum immunoglobulin IgG, IgA, and IgM levels were significantly increased. This feces enterococci colonization sites accounted for most of the digestive tract of the sow, which act directly on the host's immune system to induce intestinal immunity and stimulate the development of the thymus, spleen and bursa and other immune organs, promote macrophage cell viability or play an adjuvant role in the activation of intestinal mucosa-associated lymphoid tissue, so that SIgA and IgG secretion, enhance local immune response of the host defense-related intestinal mucosa. With the increase in sow colostrum immunoglobulin content of piglets by passive immunization complement and antibodies obtained increases, the passive immunization of newborn piglets will enhance disease resistance to infection, but also reduce the incidence of some diseases heavy. Use the following formula to calculate the rate Chuhuo earners, average birth weight, weaning weight and average weaning survival. Live Aberdeen rate (%) = number born alive / litter size × 100 = average birth weight of newborn litter weight / average number of pigs per litter weaning weight = weaning weight / litter number of pigs weaned weaning survival rate (% 0,1,3,5 days) = the number of piglets weaned / litter size × 100 1.5 colostrum samples collected after farrowing 3rnl milk samples were collected from different nipple each sow. After sample collection, centrifugal separation of whey and placed at -20 ℃ under test. Whey IgA, IgG and IgM was measured by ELISA kit operating instructions. 1.6 statistical test data "mean ± standard deviation," said the results of the use of Excel and SPSS14.0 statistical software version in the process of One-Way ANOVA analysis of variance, P <0.05 was considered significant. 2 Results 2.1 faecalis impact on sow performance can be seen from Table 1, in this experiment, sow dietary feces enterococci, their number born alive, the average birth weight, weaning weight and were significantly increased compared to the control group (P <0.05). At the end of the test sows weaning piglet weaning weight and average weaning weight than the control group increased by 22.70% and 5.30%, weaning survival rate than the control group, the experimental group improved 5.56%. Table 1 in Enterococcus faecalis sow performance? Control group test group litter size (head) 10.76 ± 2.98 11.95 ± 2.57 litter size (head) 9.91 ± 2.42a 11.28 ± 2.65b live litter rate (%) 92.10a 94.39b litter weight at birth (kg) 14.80 ± 3.78 15.94 ± 3.25 average birth weight (kg) 1.55 ± 0.23a 1.67 ± 0.24b weaned (head) piglets 9.34 ± 0.62 10.95 ± 0.94 weaning weight (kg) 54.01 ± 13.22a 66.27 ± 9.94b average weaning weight (kg) 5.85 ± 0.51a 6.16 ± 0.67b especially for the invasion of intestinal diseases and intestinal microbes play a partial protective effect. Reduce, increase immunity in piglets incidence in this study from Aberdeen live rates, average weaning weight and weaning survival rate improved significantly reflected. Conclusions The results showed that: in sow diets appropriate amount of E. faecalis can increase the survival rate of sows farrowing, weaning improve the survival rate, improve sow milk immunoglobulin content and enhance disease resistance, reducing stress damage to the body.